Isotope Laboratory Prague

Isotope Laboratory, Institute of Experimental Botany of the Czech Academy of Sciences

The Isotope Laboratory has a long-standing tradition in radiochemistry and isotope applications in plant biology. Its roots reach back to the former Institute of Nuclear Biology and Radiochemistry, originally based in Prague. Following the reorganization of research institutes in 1993, a major part of the team and expertise was integrated into the Institute of Experimental Botany of the Czech Academy of Sciences, where the laboratory has continued to develop and support isotope-based research ever since.

autoradiography
Figure 1: Visualization of tritium labelled hormone in wheat by autoradiography.

Research-grade synthesis and isotope labeling

We specialize in the synthesis of plant growth regulators and related bioactive molecules, with particular expertise in cytokinins and auxins, including site-specific tritium (³H) labeling at defined carbon positions. Our synthetic work is designed to meet the requirements of modern biochemical and physiological studies, enabling precise tracing (fig. 1), metabolic profiling, receptor/transport studies, and quantitative analytics.

Custom labeled compounds and tailored solutions

In addition to standard offerings, we provide custom synthesis of compounds labeled with ³H and/or ¹⁴C, as well as the preparation of deuterium-labeled (²H) standards for LC–MS/MS method development, stable-isotope dilution assays, and metabolism studies. Each project is approached individually, with emphasis on chemical identity, labeling strategy, stability, and suitability for the intended application.

Quality, documentation, and scientific partnership

Our laboratory operates with a strong focus on scientific rigor and reproducibility. Deliverables are accompanied by appropriate analytical documentation (e.g., NMR, MS, radio-TLC/HPLC where applicable) and consultation on labeling strategy and experimental design. We collaborate with academic and industrial partners, offering a flexible and confidential service model for both research and applied development.Didn’t find what you’re looking for? Contact us—many compounds can be prepared on request, including tailored isotope placement and specific activity requirements.